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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in lots of tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant purpose in marketing cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two extra splice variants were being documented but detailed nucleotide sequence facts just isn't but available.
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Blocking peptides are peptides that bind exclusively into the goal antibody and block antibody binding. These peptide commonly incorporates the epitope acknowledged because of the antibody.
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This tutorial outlines even further attainable optimization of cellular and immunoassay parameters to ensure the get more info very best benefits are...
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This guideline outlines further attainable optimization of cellular and immunoassay parameters to be certain the very best results are...
Antibodies bound to the blocking peptide now not bind for the epitope around the target protein. By evaluating the staining from your blocked antibody compared to the antibody by itself, you can see which staining is certain.
Goal Data Cyclin E belongs to your really conserved cyclin relatives, whose customers are characterised by a spectacular periodicity in protein abundance with the mobile cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show distinctive expression and degradation designs which contribute to the temporal coordination of every mitotic occasion. Cyclin E types a complex with and capabilities as a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S changeover. This protein accumulates with the G1-S period boundary and it is degraded as cells progress via S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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